{"id":87,"date":"2017-08-10T15:04:01","date_gmt":"2017-08-10T19:04:01","guid":{"rendered":"http:\/\/casgroups.case.edu\/cwruecs\/?page_id=87"},"modified":"2017-08-10T15:39:14","modified_gmt":"2017-08-10T19:39:14","slug":"events_20161107_liu","status":"publish","type":"page","link":"https:\/\/casgroups.case.edu\/cwruecs\/events_20161107_liu\/","title":{"rendered":"Events: Dr. Liu November 7th, 2016"},"content":{"rendered":"

Professor C.C. Liu, Wallace R. Persons Professor of Sensor Technology and Control of Case Western Reserve University, presented “Single-Use Disposable Electrochemical Label-Free Immunosensor for Dectection of Glycated Hemoglobic (HbA1c) Using Differential Pulse Voltammetry” on November 7th, 2016.<\/p>\n

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Abstract:
\nA single-use disposable in-vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. The biosensor has a three-electrode configuration: 10 nm thin gold film working and counter electrodes and a thick-film printed Ag\/AgCl refer-ence electrode which was fabricated on a polyethylene terephthalate (PET) sub-strate. Micro-fabrication techniques including sputtering vapor deposition, laser ablation and thick film printing were used in manufacturing process. This was a roll-to-roll cost effective manufacturing process making the single use, disposa-ble in vitro HbA1c biosensor a reality. Self-assembled monolayers of 3-Mercaptopropionic acid (MPA) was employed to covalently immobilize anti-HbA1c on the surface of gold electrode. A carbodiimide conjugation approach using N-(3-dimethylaminopropyl)-N\u2019-ethylcarbodiimide hydrochloride (EDC) and N\u2013hydroxysuccinimide (NHS) was undertaken for cross-linking anti-HbA1c to the carboxylic groups on one end of the MPA. Electrochemical Impedance Spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS) confirmed the excellent coverage of MPA-SAM and upward orientation of carboxylic groups. The hindering effect of HbA1c on ferricyanide\/ferrocyanide electron transfer reaction was exploited as HbA1c detection mechanism. The biosensor showed a linear range of 7.5-25 \u03bcg\/mL of HbA1c in 0.1M PBS. Using undiluted human serum as the test medium, the biosensor presented an excellent linear behavior (R2=0.999) in the range of 0.1-0.25 mg\/mL of HbA1c. The potential application of this biosensor for in vitro measurement of HbA1c for diabetic management was demonstrated.<\/p>\n","protected":false},"excerpt":{"rendered":"

Professor C.C. Liu, Wallace R. Persons Professor of Sensor Technology and Control of Case Western Reserve University, presented “Single-Use Disposable Electrochemical Label-Free Immunosensor for Dectection of Glycated Hemoglobic (HbA1c) Using Differential Pulse Voltammetry” on November 7th, 2016.<\/p>\n

\u00a0 <\/p>\n

Abstract:
\nA single-use disposable in-vitro electrochemical immunosensor for the detection of HbA1c in undiluted human serum using differential pulse voltammetry (DPV) was developed. The biosensor has a three-electrode configuration: 10 nm thin gold film working and counter electrodes and a thick-film printed Ag\/AgCl refer-ence electrode which was fabricated on a polyethylene terephthalate (PET) sub-strate. Micro-fabrication techniques including sputtering vapor deposition,<\/p>\n

Continue reading… Events: Dr. Liu November 7th, 2016<\/span><\/a><\/p>\n","protected":false},"author":19,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"spay_email":""},"_links":{"self":[{"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/pages\/87"}],"collection":[{"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/comments?post=87"}],"version-history":[{"count":7,"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/pages\/87\/revisions"}],"predecessor-version":[{"id":95,"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/pages\/87\/revisions\/95"}],"wp:attachment":[{"href":"https:\/\/casgroups.case.edu\/cwruecs\/wp-json\/wp\/v2\/media?parent=87"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}