{"id":1977,"date":"2010-05-01T20:50:09","date_gmt":"2010-05-01T20:50:09","guid":{"rendered":"http:\/\/casgroups.case.edu\/physics-senior-projects\/?p=1977"},"modified":"2017-01-24T21:17:55","modified_gmt":"2017-01-24T21:17:55","slug":"role-of-cellular-mg2-in-modulating-collagen-deposition-and-interleukin-release-in-ito-and-kupffer-cells","status":"publish","type":"post","link":"https:\/\/casgroups.case.edu\/physics-senior-projects\/role-of-cellular-mg2-in-modulating-collagen-deposition-and-interleukin-release-in-ito-and-kupffer-cells\/","title":{"rendered":"Role of Cellular Mg2+ in Modulating Collagen Deposition and Interleukin Release in Ito and Kupffer Cells"},"content":{"rendered":"<h3 class=\"style7\" align=\"center\"><span class=\"style9\">Steven Ewart with Andrea Romani (<em>SOM, Dept. of Physiology &amp; Biophysics<\/em>)<\/span><\/h3>\n<h3 class=\"style7\" align=\"center\"><a class=\"style11\" href=\"https:\/\/artscimedia.case.edu\/wp-content\/uploads\/sites\/176\/2010\/05\/15033321\/StevenEwartPoster.ppt\">Role of Cellular Mg2+ in Modulating Collagen Deposition and Interleukin Release in Ito and Kupffer Cells<\/a><\/h3>\n<p align=\"justify\"><em>Introduction<\/em>: Magnesium ion (Mg2+) is the second most abundant cation after potassium within mammalian cells, including liver cells. At the cellular level, Mg2+ has been reported to regulate more than 180 enzymes, especially those involved in cell cycle regulation and glucose metabolism. Acute and chronic ethanol administration reduces total Mg2+ content in liver tissue. A decrease in cellular Mg2+ content has been associated with increased pro-inflammatory cytokines release and altered collagen deposition.<\/p>\n<p align=\"justify\"><em>Hypothesis:<\/em> In the present study I will test the hypothesis that a reduced cellular Mg2+ content within Kupffer and Ito (stellate) cells is sufficient to generate an increased production of Il-2 and Il-6 and an increased collagen deposition, respectively, or it predisposes to these changes in the presence of ethanol administration.<\/p>\n<p align=\"justify\"><em>Methods:<\/em> Kupffer and Ito cells isolated from rat livers will be maintained in culture in the presence of varying extracellular Mg2+ concentrations (i.e. 0.4, 0.8 and 2 mM). Cell cultures will be challenged by addition of different doses of ethanol ranging from 0.01% to 1% for varying period of time spanning from 15 min to 96 hours. Cellular Mg2+ content will be measured by atomic absorbance spectrophotometry (AAS). Release of IL-2 and IL-6 in the extracellular medium will be assessed by Elisa assay. Collagen deposition will be determined by 3H-proline incorporation. mRNA levels for Il-2, Il-6 and collagen will be determined by RT-PCR.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Steven Ewart with Andrea Romani (<em>SOM, Dept. of Physiology &amp; Biophysics<\/em>)<br \/>\n<a class=\"style11\" href=\"https:\/\/artscimedia.case.edu\/wp-content\/uploads\/sites\/176\/2010\/05\/15033321\/StevenEwartPoster.ppt\">Role of Cellular Mg2+ in Modulating Collagen Deposition and Interleukin Release in Ito and Kupffer Cells<\/a><\/p>\n<p align=\"justify\"><em>Introduction<\/em>: Magnesium ion (Mg2+) is the second most abundant cation after potassium within mammalian cells, including liver cells. At the cellular level, Mg2+ has been reported to regulate more than 180 enzymes, especially those involved in cell cycle regulation and glucose metabolism. Acute and chronic ethanol administration reduces total Mg2+ content in liver tissue. A decrease in cellular Mg2+ content has been associated with increased pro-inflammatory cytokines release and altered collagen deposition.<\/p>\n<p><a href=\"https:\/\/casgroups.case.edu\/physics-senior-projects\/role-of-cellular-mg2-in-modulating-collagen-deposition-and-interleukin-release-in-ito-and-kupffer-cells\/\" class=\"more-link\">Continue reading&#8230; <span class=\"screen-reader-text\">Role of Cellular Mg2+ in Modulating Collagen Deposition and Interleukin Release in Ito and Kupffer Cells<\/span><\/a><\/p>\n","protected":false},"author":19,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"spay_email":""},"categories":[48,1],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/1977"}],"collection":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/comments?post=1977"}],"version-history":[{"count":3,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/1977\/revisions"}],"predecessor-version":[{"id":3028,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/1977\/revisions\/3028"}],"wp:attachment":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/media?parent=1977"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/categories?post=1977"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/tags?post=1977"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}