{"id":2538,"date":"2009-05-01T21:10:54","date_gmt":"2009-05-01T21:10:54","guid":{"rendered":"http:\/\/casgroups.case.edu\/physics-senior-projects\/?p=2538"},"modified":"2016-06-20T14:33:26","modified_gmt":"2016-06-20T14:33:26","slug":"nanometer-optical-imaging-of-fluorescent-dyes","status":"publish","type":"post","link":"https:\/\/casgroups.case.edu\/physics-senior-projects\/nanometer-optical-imaging-of-fluorescent-dyes\/","title":{"rendered":"Nanometer Optical Imaging of Fluorescent Dyes"},"content":{"rendered":"<h3 align=\"center\">Matthew Johns with Rolfe Petschek<\/h3>\n<h3 align=\"center\"><a href=\"\/physics-senior-projects\/files\/2009\/05\/MatthewJohnsPoster.pptx\">Nanometer Optical Imaging of Fluorescent Dyes<\/a><\/h3>\n<p align=\"justify\">If a single fluorescent dye molecule is near the image plane of a far-field microscope, it is possible, by carefully analyzing the image of the particle in an ordinary light microscope, to find the position of the particle in the directions perpendicular to the axis of the microscope to accuracy significantly better than the wavelength of light that is the \u201cRayleigh limit.\u201d\u00a0 We will examine theoretically how these limits can be improved by interfering light on different paths or with different polarizations, and then examining simultaneously the images that these interferences form.\u00a0 Various schemes for making these interferences will be examined to find which will result in the best signal to noise for various combinations of the 3-dimensional position of the particle and its orientation.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Matthew Johns with Rolfe Petschek<a href=\"\/physics-senior-projects\/files\/2009\/05\/MatthewJohnsPoster.pptx\">Nanometer Optical Imaging of Fluorescent Dyes<\/a><\/p>\n<p align=\"justify\">If a single fluorescent dye molecule is near the image plane of a far-field microscope, it is possible, by carefully analyzing the image of the particle in an ordinary light microscope, to find the position of the particle in the directions perpendicular to the axis of the microscope to accuracy significantly better than the wavelength of light that is the \u201cRayleigh limit.\u201d\u00a0 We will examine theoretically how these limits can be improved by interfering light on different paths or with different polarizations, and then examining simultaneously the images that these interferences form.\u00a0<\/p>\n<p><a href=\"https:\/\/casgroups.case.edu\/physics-senior-projects\/nanometer-optical-imaging-of-fluorescent-dyes\/\" class=\"more-link\">Continue reading&#8230; <span class=\"screen-reader-text\">Nanometer Optical Imaging of Fluorescent Dyes<\/span><\/a><\/p>\n","protected":false},"author":19,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"spay_email":""},"categories":[81,41],"tags":[],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/2538"}],"collection":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/users\/19"}],"replies":[{"embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/comments?post=2538"}],"version-history":[{"count":2,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/2538\/revisions"}],"predecessor-version":[{"id":2730,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/posts\/2538\/revisions\/2730"}],"wp:attachment":[{"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/media?parent=2538"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/categories?post=2538"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/casgroups.case.edu\/physics-senior-projects\/wp-json\/wp\/v2\/tags?post=2538"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}